36th Annual Student Research Conference
The role of MRP-5 exporting heme from the hypodermis in development of adult Caenorhabditis elegans
Manie Brema
Dr. Stephanie Maiden, Faculty Mentor
Heme is an essential molecule for supplying oxygen throughout the body and energy for metabolism. Caenorhabditis elegans obtained heme from their environment and it’s transported by MRP-5. MRP-5 gene is expressed in the intestine's embryonic development. mrp-5 is also expressed in the epidermis, however, few studies are done to show its effect on epidermal development in C.elegans. This study aims to examine epidermal defects in C.elegans using RNAI-specific methods and microscopy. From the data obtained, epidermal defects were not present in epidermal-specific RNAi for mrp-5, but it did exhibit slow growth in the larval stages of worms and significant change in embryonic lethality as seen with worm strains. This result indicates that mrp-5 might not have a strong knockdown. For future direction, we will replicate the experiment with a large sample and use the injection method for stronger knockout significant differences in epidermal development.
Keywords: c. elegans , epidermis , MRP-5, heme, development
Topic(s):Biology
Biochemistry and Molecular Biology
Presentation Type: Oral Presentation
Session: 108-4
Location: MG 1000
Time: 9:15