30th Annual Student Research Conference
Isolation and Elucidation of a Small Sollasin from the Sponge Pachastrellidae: Inhibitor of RAGE
Lydia J. Davis
Dr. Timothy D. Walston, Faculty Mentor
A sollasin related compound has been isolated from a sponge in the family Pachastrellidae. The chemical structure of this molecule was elucidated using spectroscopic analysis with the following instrumentation: HPLC, MPLC, MS, prep-LCMS, 1D and 2D NMR. This compound was tested in a RAGE bioassay using the PANC-1 pancreatic cancer cell line to determine its overall activity in inhibiting both membrane bound and membrane soluble RAGE. The chemical exhibited promise as a potential candidate for RAGE inhibition when a flow cytometric analysis of intracellular RAGE was performed; moderate RAGE inhibition was observed. A CYTO_ID assay was also employed; results indicated that the compound inhibited autophagy within the cancer cells under study.
Keywords: Natural Products Chemistry, Marine Biology, Pancreatic Cancer, Cancer Research, NMR, LCMS, HPLC, MS
Topic(s):Chemistry
Biology
Interdisciplinary
Presentation Type: Poster
Session: 7-
Location: GEO - SUB
Time: