23rd Annual Student Research Conference
Effective Extraction of High Amylose Starch through the Use of a Novel Method of Dimethyl Sulfoxide (DMSO) Leachate
Samah Hassan A. Hassan* and Suzanne N. Pitts
Dr. Mark Campbell, Faculty Mentor
The plant breeding program at Truman focuses on the development of high-amylose corn possessing Amylomaize VII starch. Amylomaize VII starch is made of 70% amylose and is used for manufacturing biodegradable plastics and as a pre-biotic food additive. Our goal was to examine a more rapid starch extraction technique in order to expedite the genetic selection process. Conventional laboratory wet-milling is labor intensive since breeding programs require screening large sample sizes. A method was examined using dimethyl sulfoxide (DMSO) to soak maize kernels to leach starch followed by starch precipitation with ethanol and compared to starch prepared conventionally. This study is very significant because it has the possibility of actually increasing the progress of our collaborative breeding project with the USDA - GEM project.
Keywords: Amylose, AmylomaizeVII, GEM, Dimethyl Sulfoxide, Pre-Biotic
Topic(s):Agricultural Science
Biology
Chemistry
Presentation Type: Poster
Session: 6-4
Location: SUB-GEO
Time: 4:15