2010 Student Research Conference:
23rd Annual Student Research Conference

Evaluation of marker assisted backcross selection for the development of high amylose Corn
Avinash Karn
Dr. Mark Campbell, Faculty Mentor

High amylose corn has numerous food and industrial applications such as biodegradable plastics, adhesives and as a prebiotic to improve digestive human health. Studies at Truman and SDSU have revealed that a novel recessive starch branching enzyme 1a (sbe1a) likely plays a significant role in increasing amylose content from 50% to at least 70% in the variety GEMS-0067 developed at Truman. Our projects current focus is to continue examining this putative novel sbe1a allele using SSR molecular markers and confirm co-segregation with the amylose phenotype determined by wet-chemistry method using genetically diverse maize lines from the USDA GEM project. A data base has been initiated to examine fragment lengths of SSR markers closely linked to the target which involves PCR amplifications and gel electrophoresis of the donor line (GEMS-0067) and 30 high yielding recipient GEM lines. At this point a number of polymorphic markers have been identified within these materials and will be examined for their potential application for marker assisted backcrossing

Keywords: high amylose corn, sbe1a, Gems-0067, SSR molecular markers, USDA GEM ARS, biodegradable, prebiotic, back crossing

Topic(s):Agricultural Science

Presentation Type: Poster

Session: 6-2
Location: SUB-GEO
Time: 4:15

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