18th Annual Student Research Conference
Science
DNA Sequence Diversity at a SHAGGY-like Gene among Inbred Lines and Open-Pollinated Landraces of Maize
Benjamin A. Schmidt
Dr. Brent Buckner, Dr. Diane Janick-Buckner, and Dr. Patrick S. Schnable (Iowa State University), Faculty Mentors
The SHAGGY kinase gene family has been studied extensively in Drosophila and is implicated in various signal transduction pathways in both Drosophila and in mammals. In plants SHAGGY-like kinases are present as small gene families – ten have been characterized in Arabidopsis. Using the Arabidopsis SHAGGY kinase 11 (ASK11) protein sequence as a query, maize sequence databases were mined in order to identify ESTs and genomic sequences which encode SHAGGY kinase-like proteins. Those with the greatest similarity to ASK11 were used to design oligonucleotide primers which during the polymerase chain reaction amplified both exonic and predicted intronic sequences. DNA was amplified, cloned, and sequenced from five North American inbred lines to examine sequence diversity at this locus. In addition, two sequence variants of a SHAGGY-like sequence amplified using a different primer differing only in the placement of a 12 bp sequence within an intron, were identified in inbred line B73.
Keywords: SHAGGY, Genetic Diversity, Maize, Zea mays
Topic(s):Biology
Presentation Type: Poster
Session: 29-66
Location: OP Lobby & Atrium
Time: 1:15