18th Annual Student Research Conference
Science
Proteins and Viral RNA Associated with the Gag-Ubc9 Complex of Mason-Pfizer Monkey Virus Infected HOS Cells
Kimberly J. Kennett
Dr. Robert Weldon, Jr. (University of Nebraska-Lincoln) and Dr. Michael Lockhart, Faculty Mentors
The viral protein Gag and the cellular nuclear pore associated protein hUbc9 have been shown to colocalize in the cytoplasm and near the nuclear membrane of COS-1 and HeLa cells infected with Mason-Pfizer monkey virus (MPMV) (Weldon et al., 2003). Because Gag plays a number of roles in the retrovirus life cycle, its association with a cellular protein may indicate host cell involvement in the proliferation of retroviruses. The aim of this study was to isolate the Gag-Ubc9 complex of MPMV infected HOS cells via immunoprecipitation and identify any viral RNA and/or proteins associated with the Gag-Ubc9 complex associated with Gag through reverse transcriptase polymerase chain reaction (RT-PCR) and sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This project was initiated in the summer of 2004 under the mentorship of Dr. Robert Weldon, Jr. at the Nebraska Center for Virology as part of the Undergraduate Student Research Experience in Virology (USREV) program.
Keywords: Mason-Pfizer , monkey virus, HOS cells, Ubc9, Gag, retrovirus
Topic(s):Biology
Presentation Type: Poster
Session: 29-69
Location: OP Lobby & Atrium
Time: 1:15