Hog Manure Extract and Porcine Pancreatic Elastase Modulate
Airway Epithelial Cell EGF Receptors, Calcium Signaling, and
Cytokine Release
Audrey C. Zimbelman
Dr. Myron L. Toews (University of Nebraska - Medical Center) and Dr. Susan Guffey, Faculty Mentors
Workers in swine confinement facilities develop chronic inflammatory lung disease, an aqueous hog-barn dust extract (HDE) induces rapid (15min) and slower (18hr) decreases in cell surface EGF receptor (EGFR) binding, which are involved in HDE stimulation of IL6/8 release. We hypothesized that components from hog manure would also alter EGFRs and contribute to the effects of HDE. Treatment of BEAS-2B cells with lyophilized manure extract (LME) induced decreases in EGFR binding at 18hr, but LME did not mimic the 15min decrease EGFR binding seen with HDE. LME stimulated IL6/8 release and intracellular Ca2+ mobilization as did HDE. Porcine pancreatic elastase (PPE) was identified in HDE, PPE stimulated IL6/8 release and decreased EGFR binding at 18hr but not 15min, similar to LME. These data indicate that portions of the disease-relevant activity of HDE likely derives from hog manure and PPE may be one of the active components of LME and HDE.
Keywords: Swine Confinement Facilities, Chronic Inflammatory Lung Disease, hog-barn dust extract, BEAS-2B human airway epithelial cells, Cell surface EGFR Binding, Hog Manure, Porcine Pancreatic Elastase
Topic(s):Biology
Chemistry
Presentation Type: Oral Paper
Session: 58-5
Location: MG 2001
Time: 3:45