35th Annual Student Research Conference
Determination of the Ratio of Oxidized Cysteine to G-S
Alison Regina
Dr. Cassidy Dobson, Faculty Mentor
?-S is a protein that aggregates to form cataracts in the eye lens. Cystine, an oxidizing agent, could potentially help undo the aggregation process due to the extra cysteines located on ?-S. Potential experiments include incubating DBF (disulfide bond-forming enzyme) and cystine with ?-S to see how effective cystine is at oxidizing DBF, which acts as a molecular chaperone to mediate protein refolding.
Current experimentation includes incubating various ratios of ?-S and cystine to determine the ratio of ?-S and cystine necessary to facilitate the refolding process. ?-S and some buffer were used as the control and also incubated along with the other samples. The projected results are that a 5 to 1 ratio or less of cystine to ?-S will be able to appear on gels and will be enough to oxidize ?-S based on previous experiments.
Keywords: G-S, DBF, cystine, cysteine, oxidizing agent
Topic(s):Chemistry
Biochemistry and Molecular Biology
Biology
Presentation Type: Oral Presentation
Session: 205-4
Location: MG 2001
Time: 11:00