18th Annual Student Research Conference
Human Potential and Performance
Establishing an Assay for Screening Compounds that Inhibit ebola Glycoprotein-pseudotyped Retovirus Entry
Elisea E. Avalos* and Jose A. A. Gonzalez Feliciano
Dr. Jerry Mayhew, Faculty Mentor
In this study a pseudotyped virus that contains the protein core from the Moloney Murine Leukeamia virus (Mo-MuLV) and displays the Ebola glycoprotein (Zaire subtype) on its envelope was used. The goal of the project was to discover compounds that inhibit viral entry. We wished to establish an assay that would allow us to see a reduction of fluorescence after viral transduction if the compound was able to inhibit the entry of the virus. By observation of the absorption and emission spectra of the Aequorea victoriaa and Renilla renifromis GFPs, it was found that the A.victoria GFP is more appropriate to use in teh fluorescence reader.
Keywords: Ebola, Aequorea victoria, viral, pseudotyped virus, virus
Topic(s):Health Science
Presentation Type: Poster
Session: 29-43
Location: OP Lobby & Atrium
Time: 1:15