Comparative Study of Lepidopteran Resistance in Maize Lines through Protein Analysis
Emily C. Dunn
Dr. Brent Buckner and Dr. Georgia E. Davis (University of Missouri-Columbia), Faculty Mentors
To date, several quantitative trait loci (QTL) corresponding to resistance from damage during the whole-stage by Lepidopteran have been identified and protein patterns from resistant (Mp705) and susceptible (Oh28) parental lines were analyzed through 2-demensional gel electrophoresis. In order to narrow the number of proteins found, the midgut of fall armyworm (FAW) and southwestern corn borer (SWCB) exposed to resistant (Mp705) and susceptible (Va35) lines through artificial diet was extracted and analyzed for protein content. In addition preference tests were run to see if the Lepidopteran were able to sense the resistance factors in the tissue before feeding on it. The resulting data showed that Glossy15 and Glossy8 are genes that are directly correlated with Lepidopteran resistance. These genes can then be integrated into maize lines in order to reduce the Lepidopteran damage as well as reduce the cost of pesticides.
Keywords: Glossy8, Glossy15, maize, resistance, Lepidopteran, fall armyworm, southwestern, corn borer
Topic(s):Biology
Presentation Type: Poster
Session: 26-37
Location: OP Lobby & Atrium
Time: 1:15