19th Annual Student Research Conference
Science
Replication of Retroviruses: Construction of a Nup133 cDNA Vector
Kristi M. Teal
Dr. Robert A. Weldon (University of Nebraska-Lincoln) and Dr. Michael Lockhart, Faculty Mentors
Retroviral replication is dependant upon the virus’s ability to integrate its genetic material into the host’s DNA and the subsequent production of proteins. An essential step in replication of these viruses is nuclear transport. Bidirectional nuclear transport is mediated by nuclear pore complexes, embedded in the nuclear envelope, that are made up of approximately 30-50 different nucleoporin proteins (Nup). The main focus of this study was to determine whether Nup 133 is required for, or associates with, viral replication and if so how Nup 133 interacts in the viral replication process. In order to address these questions, the full length cDNA sequence of Nup 133 needed to be isolated and transformed into a vector that would allow further experimentation. My project for the summer attempted to produce a construct that contained the full length sequence of Nup133. Then the full length sequence would be sub-cloned into a myc-epitope plasmid for use in further testing.
Keywords: Retrovirus, Nuclear pore complex, Nup133
Topic(s):Biology
Presentation Type: Poster
Session: 60-64
Location: OP Lobby and Atrium
Time: 4:15