2022 Student Research Conference:
35th Annual Student Research Conference

Determination of the Ratio of Oxidized Cysteine to G-S

Alison Regina
Dr. Cassidy Dobson, Faculty Mentor

 ?-S is a protein that aggregates to form cataracts in the eye lens. Cystine, an oxidizing agent, could potentially help undo the aggregation process due to the extra cysteines located on ?-S.  Potential experiments include incubating DBF (disulfide bond-forming enzyme) and cystine with ?-S to see how effective cystine is at oxidizing DBF, which acts as a molecular chaperone to mediate protein refolding. 

Current experimentation includes incubating various ratios of ?-S and cystine to determine the ratio of ?-S and cystine necessary to facilitate the refolding process. ?-S and some buffer were used as the control and also incubated along with the other samples. The projected results are that a 5 to 1 ratio or less of cystine to ?-S will be able to appear on gels and will be enough to oxidize ?-S based on previous experiments. 


Keywords: G-S, DBF, cystine, cysteine, oxidizing agent

Biochemistry and Molecular Biology

Presentation Type: Oral Presentation

Session: 205-4
Location: MG 2001
Time: 11:00

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