35th Annual Student Research Conference
Reduction of Methanol-Containing Hazardous Waste Produced By the Western Blot Method
Keira Sprogoe
Dr. Daniela Ostrowski, Faculty Mentor
The Western Blot method is a protein analysis technique utilized in cell and molecular biology. The transfer of proteins following gel electrophoresis to a protein-binding membrane allows for the antibody staining and detection of specific proteins. Methanol is commonly added to the transfer buffer (10 - 20%) to promote protein binding to the membrane. Unfortunately, methanol has adverse effects on the environment and is toxic to humans, causing significant mortality and morbidity. In addition, hazardous waste disposal is very costly for research labs and universities. Reducing the use of methanol will benefit the environment, protect researchers, and minimize costs. My study aims to compare the quality of protein transfer in the Western Blot method between the use of transfer buffers with and without methanol.
Keywords: Western Blot, protein, methanol, transfer buffer, hazardous waste
Topic(s):Biochemistry and Molecular Biology
Biology
Presentation Type: Poster Presentation
Session: 2-17
Location: SUB Activities Room
Time: 3:00