2021 Student Research Conference:
34th Annual Student Research Conference

Optimization of the Aggregation of Gamma D Crystallin Protein


Cade K. Rohler
Dr. Cassidy Dobson, Faculty Mentor

Gamma D crystallin is a human protein commonly found in the human eye. The aggregation of which is a leading cause of cataracts. This work focuses on the optimization of aggregation conditions for gamma D crystallin in order to investigate the effect of molecular chaperones on the reversal and prevention of the aggregation. By optimizing the aggregation conditions a reliable production of gamma D crystallin aggregate can be achieved. This work focused on the aggregation of gamma D crystallin via oxidation in the presence of chemical oxidizers. Results were confirmed through the use of SDS PAGE. We were able to identify a chemical oxidant capable of reliably producing gamma D aggregates. Future work will focus on optimizing other aggregation conditions such as molar ratios and incubation times.

Keywords: Gamma Crystallins, SDS PAGE, Cataracts, Protein Aggregation

Topic(s):Chemistry
Biochemistry and Molecular Biology

Presentation Type: Asynchronous Virtual Oral Presentation

Session: 3-18
Location: https://flipgrid.com/f86d186b
Time: 0:00

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