2005 Student Research Conference:
18th Annual Student Research Conference

Human Potential and Performance

Establishing an Assay for Screening Compounds that Inhibit ebola Glycoprotein-pseudotyped Retovirus Entry
Elisea E. Avalos* and Jose A. A. Gonzalez Feliciano
Dr. Jerry Mayhew, Faculty Mentor

In this study a pseudotyped virus that contains the protein core from the Moloney Murine Leukeamia virus (Mo-MuLV) and displays the Ebola glycoprotein (Zaire subtype) on its envelope was used. The goal of the project was to discover compounds that inhibit viral entry. We wished to establish an assay that would allow us to see a reduction of fluorescence after viral transduction if the compound was able to inhibit the entry of the virus. By observation of the absorption and emission spectra of the Aequorea victoriaa and Renilla renifromis GFPs, it was found that the A.victoria GFP is more appropriate to use in teh fluorescence reader.

Keywords: Ebola, Aequorea victoria, viral, pseudotyped virus, virus

Topic(s):Health Science

Presentation Type: Poster

Session: 29-43
Location: OP Lobby & Atrium
Time: 1:15

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* Indicates the Student Presenter
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