Optimizing the Aggregation of Gamma S Crystallin

Reuben G. Kern
Dr. Cassidy Dobson, Faculty Mentor

Gamma crystallins are proteins found in the eye that can aggregate to form cataracts. In order to study how these proteins aggregate together, we need to be able to create aggregates in vitro. Our research focused on optimizing the aggregation of gamma S crystallin via oxidation. Variables such as amount of time incubated, identity of the oxidizing agent, and optimal ratio of protein to oxidant were tested using polyacrylamide gel electrophoresis and size exclusion chromatography. We were able to optimize the procedure to produce the most aggregate, as well as learn about how crystallin aggregates are formed. Future work will classify how a known disulfide protein chaperone can affect gamma crystallin aggregates in order to prevent or reverse cataracts

Keywords: gamma crystallins, protein aggregation, SDS-PAGE

Topic(s):Biochemistry and Molecular Biology

Presentation Type: Oral Presentation

Session: TBA
Location: TBA
Time: TBA