2018 Student Research Conference:
31st Annual Student Research Conference

The role of UNC-33/CRMP in the Caenorhabditis elegans embryonic epidermis


Rachel Prins* and Peter K. Windsor
Dr. Stephanie L. Maiden and Dr. Bill Miller, Faculty Mentors

UNC-33 is a homolog of the mammalian collapsin response mediator protein (CRMP) in Caenorhabditis elegans with a known role in neuronal development. Mutations in the unc-33 gene of C. elegans have been proposed present severely uncoordinated movement and abnormalities in the dynamic process of microtubule formation. We identify how changes in amino acid sequences alter the function of CRMP homologs, how that leads to structural defects in microtubule formation, and the affects on cellular function and physical development of C. elegans. Using DIC microscopy analysis of the major developmental stages we were able to show that epidermal morphogenesis exhibited developmental delays in mutant strains indicating defects in the interactions of CRMP mediator proteins with microtubule formation in the embryonic epidermis. The second level of analysis involved identifying the DNA defect in two mutant variants that have observable physical defects but unknown ‘lesions’ in the unc-33 gene through TOPO-TA sequencing.

 

Keywords: unc-33, C. elegans

Topic(s):Biology
Chemistry

Presentation Type: Oral Paper

Session: 103-4
Location: MG 1096
Time: 8:45

Add to Custom Schedule

* Indicates the Student Presenter
Contact SRC Webmaster  |  SRC Privacy Policy