2015 Student Research Conference:
28th Annual Student Research Conference

The Bioinformatic Analysis of Mutant raggedseedling378 in Maize Leaf Development.
Rosalyn C. Henn* and Marissa N. Fabbri
Dr. Diane Janick-Buckner and Dr. Brent Buckner, Faculty Mentors

Recessive maize raggedseedling-378 (rgd378) mutants are very small, develop filamentous leaves and are highly susceptible to fungal infection. We performed bulked segregant RNA-seq analysis on shoot tissue of 7 day old rgd378 and wild-type sibling plants to locate the chromosomal interval in which the rgd378 gene resides, as well as to perform differential gene expression analysis. The Tuxedo protocol identified over 5,400 differentially regulated genes in mutant compared to wild-type shoot tissue. Gene set enrichment analysis was carried out utilizing BiNGO and PlantGSEA to identify over-and under-represented GO categories of genes. Genes that function in glycolysis, fatty acid catabolism and responses to both abiotic and biotic stresses were over-represented in rgd378 RNA-seq samples, whereas the wild-type samples were enriched in genes that function in photosynthesis, chloroplast biogenesis, DNA replication, cell division and leaf development.

Keywords: Maize, RNA-seq, developmental mutant, genetics

Topic(s):Biology

Presentation Type: Poster

Session: 3-5
Location: GEO-SUB
Time: 3:30

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