24th Annual Student Research Conference
Breeding Specialty Starch Maize Using Exotic Genetic Resources for Gene
Discovery of Novel Alleles and Modifiers with Materials Generated from
USDA-ARS GEM Project
Avinash Karn
Dr. Mark Campbell, Dr. Brent Buckner, and Dr. Diane Janick-Buckner, Faculty Mentors
Amylomaize VII, a class of High Amylose Maize with at least 70% of the kernel starch composed of the linear amylose polymer, has had numerous food and industrial applications including the manufacturing of biodegradable plastics, adhesives and candies. It is significant source of resistant starch, a pre-biotic, that increases populations of beneficial micro-flora in the lower digestive system in humans, among them being of most interest, the suppression colorectal oncogenesis. A novel recessive starch branching enzyme 1a (sbe1a) likely plays a significant role in the presence of the ae allele in elevating amylose from ~55% to >70% in the germplasm release GEMS-0067. A series of gene specific PCR-based marker were designed in order to identify the putative sbe1a::gm67 allele in GEMS-0067, for the purpose of initiating a marker-based selection protocol to allow a more rapid conversion of GEM releases previously selected for yield and agronomics to Amylomaize VII parent lines
Keywords: high amylose corn, Plant Breeding, colon cancer, Molecular Biology, Starch, Zea mays, USDA , Agriculture
Topic(s):Agricultural Science
Biology
Presentation Type: Poster
Session: 1-2
Location: Georgian Room - SUB
Time: 4:30