2010 Student Research Conference:
23rd Annual Student Research Conference

The Effect of In Vivo and In Vitro Administration of Gossypol on p65 Expression in Dystrophic Muscle
Katherine M. Kuhn*, Elizabeth Cottey, Andrea Cottey, Casey Stefanski, Heather Curtis, Kay Bruemmer, Jenna Sesti, and Corinna A. Warnars
Dr. Emily Smith and Dr. C. George Carlson (AT Still University), Faculty Mentors

In Duchenne Muscular Dystrophy patients and a mouse model of the disease (mdx mouse), nuclear activation of NF-kB (p65, p50) is substantially elevated (Kumar and Boriek 2003). Inhibition of nuclear NF-kB activation is beneficial to dystrophic muscle (Carlson et al. 2005). Gossypol has been shown to decrease p65 expression in human leukemia cells in vitro (Moon et al. 2008). To examine whether gossypol influences p65 expression in dystrophic muscle, the drug was administered in vitro and in vivo to mdx mice and the total p65 expression was determined in costal diaphragm. Oral gavage mice and subcutaneous injection mice received a daily treatment of 10mg/kg gossypol for 10 days. Western blots of p65 expression were then obtained from whole cell, nuclear, and cytosolic extracts. Whole cell extracts were also obtained from isolated mdx diaphragms exposed in vitro to gossypol for 7 and 48 hours. Results varied depending upon treatment protocol.

Keywords: Muscular Dystrophy, NFkB, Mdx Mouse, Gossypol, Costal Diaphragm


Presentation Type: Poster

Session: 8-12
Location: SUB-GEO
Time: 4:15

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