2010 Student Research Conference:
23rd Annual Student Research Conference

Hog Manure Extract and Porcine Pancreatic Elastase Modulate Airway Epithelial Cell EGF Receptors, Calcium Signaling, and Cytokine Release
Audrey C. Zimbelman
Dr. Myron L. Toews (University of Nebraska - Medical Center) and Dr. Susan Guffey, Faculty Mentors

Workers in swine confinement facilities develop chronic inflammatory lung disease, an aqueous hog-barn dust extract (HDE) induces rapid (15min) and slower (18hr) decreases in cell surface EGF receptor (EGFR) binding, which are involved in HDE stimulation of IL6/8 release. We hypothesized that components from hog manure would also alter EGFRs and contribute to the effects of HDE. Treatment of BEAS-2B cells with lyophilized manure extract (LME) induced decreases in EGFR binding at 18hr, but LME did not mimic the 15min decrease EGFR binding seen with HDE. LME stimulated IL6/8 release and intracellular Ca2+ mobilization as did HDE. Porcine pancreatic elastase (PPE) was identified in HDE, PPE stimulated IL6/8 release and decreased EGFR binding at 18hr but not 15min, similar to LME. These data indicate that portions of the disease-relevant activity of HDE likely derives from hog manure and PPE may be one of the active components of LME and HDE.

Keywords: Swine Confinement Facilities, Chronic Inflammatory Lung Disease, hog-barn dust extract, BEAS-2B human airway epithelial cells, Cell surface EGFR Binding, Hog Manure, Porcine Pancreatic Elastase

Topic(s):Biology
Chemistry

Presentation Type: Oral Paper

Session: 58-5
Location: MG 2001
Time: 3:45

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