Aggregation of Gamma Crystallin Proteins via Chemical Modifiers

Addison M. Leabo
Dr. Cassidy Dobson, Faculty Mentor

Gamma crystallins are the major class of structural proteins in the eye. Their aggregation is the primary cause of cataracts which affects over 35 million individuals worldwide. Our lab seeks to understand the aggregation behavior of gamma crystallins and to investigate if this aggregation can be reversed or even prevented. In order to study these interactions, we must first discover the most ideal way to obtain aggregated gamma crystallins. This lab has studied multiple variables to induce aggregation, including time and chemical modifiers. Gamma crystallins do aggregate slowly over time, as confirmed by SDS-PAGE and size exclusion chromatography. We are currently seeking chemical reagents to induce aggregation quickly. Copper chloride and glutathione are both being investigated to determine which reagents most reliably and rapidly can aggregate Gamma-D crystallin, an essential step for understanding the chemical nature of cataracts.  

Keywords: gamma crystallins, protein aggregation, SDS-PAGE, biochemistry, biology

Topic(s):Biology
Chemistry

Presentation Type: Oral Presentation

Session: TBA
Location: TBA
Time: TBA