Expression of Lonesome Highway Paralogs in Maize Inbred Lines
Sarah K. Mislan
Dr. Diane Janick-Buckner and Dr. Brent Buckner, Faculty Mentors
The lonesome highway gene in Arabidopsis is involved in regulating root development and controlling cell fate decisions during xylem development. We have identified two paralogs of the lonesome highway gene in the maize genome: ZmLWHA and ZmLHWB. Using quantitative RT-PCR, the expression levels of ZmLHWA and ZmLHWB paralogs from above-ground plant tissue were examined in nine inbred maize lines. Interestingly, these maize lines showed different ratios of ZmLWHA to ZmLHWB expression. We measured the expression levels of both paralogs in additional tissues for two of these inbred lines. Expression of the ZmLWH paralogs was higher in the shoot and root tip than root tissue; additionally, the paralogs are differentially expressed in these tissues.
Keywords: Maize, paralogs, Expression
Topic(s):Biology
Presentation Type: Poster
Session: 4-5
Location: GEO-SUB
Time: 3:30