25th Annual Student Research Conference
Identifying and distinguishing between single and double mutant corn using Near Infrared Transmittance Spectroscopy
Enato A. Esangbedo♦* and Akriti Panthi
Dr. Mark Campbell, Faculty Mentor
Genetic variation in corn has brought about mutant corn endosperm genotypes with novel properties. Current methods for distinguishing corn based on their genotypes are long, tedious and involve the destruction of the kernel. The purpose of this study was to use Near Infrared Spectroscopy to identify two high amylose genotypes and determine if they could be discriminated from one another based on spectra. Near Infrared Spectra was collected from whole grain samples of corn with two different genotypes, one with recessive allele ae and the modifier genes sbe1::gems67 (novel properties of this corn include resistant starch) and the other a double mutant aewx with the sbe1::gems67 modifier gene (slowly digestable starch). If this effort is successful we would have developed a rapid, non-destructive way of determining corn genotypes for selection in our breeding project. The results of the experiments will be discussed.
Keywords: Near Infrared Spectroscopy, High amylose corn, Resistant starch, Slowly digestable starch, ae genotype, aewx genotype, Modifier gene, Classification calibrations
Topic(s):Biology
Presentation Type: Poster
Session: 200-7
Location: Georgian Room - SUB
Time: 3:30
♦ Indicates Truman Graduate Student