19th Annual Student Research Conference
Science
Global Gene Expression Patterns in the Maize Shoot Apical Meristem
Lisa D. Grantham* and Ashley N. Lough
Dr. Diane Janick-Buckner and Dr. Brent Buckner, Faculty Mentors
As part of a project aimed at understanding the cellular networks involved in shoot apical meristem function and in the formation of leaf primordia, we are analyzing the results of several microarray experiments. BLASTn and BLASTx analyses were evaluated in an attempt to provide an annotation, based on nucleic acid sequence identity and/or amino acid sequence identity/similarity, for each of several thousand differentially regulated (p-value of less than 0.0001) cDNA probes on the microarrays. In addition, EST probe sequences were used as queries of the Maize Assembled Gene Islands (MAGI) database (http://magi.plantgenomics.iastate.edu/) to identify longer ESTs or genomic sequences for which BLASTn and BLASTx could be performed and evaluated. Each differentially regulated cDNA was annotated with a gene name and placed into a functional category. To date our group has annotated over 3,500 differentially regulated EST probes. Evaluation of these data is ongoing and will be presented.
Keywords: maize, gene expression, meristem
Topic(s):Biology
Presentation Type: Poster
Session: 60-62
Location: OP Lobby and Atrium
Time: 4:15