31st Annual Student Research Conference
Function and Characterization of the yisR Gene in Bacillus subtilis
Sarah E. Evans* and Sarah M. Connolly
Dr. Joyce Patrick, Faculty Mentor
Bacillus subtilis is a model Gram-positive bacterium used to better understand other Gram-positive organisms. Past research suggested that yisR allows B. subtilis to continue growing under nutrient-deficient conditions (unpublished data). However, other data suggests that degA, a downstream gene under the same promoter as yisR is actually responsible for nutrient regulation. To investigate this further, a growth assay of a yisR knockout strain and complement strain were done under regular and nutrient deficient conditions. Additionally, yisR and yisRdegA deletions were constructed and growth assays with these strains were also performed. Current research is focused on determining the sporulation phenotype via several sporulation assays of both yisR and degA knockout strains to help determine which gene is responsible for nutrient regulation.
Keywords: Bacillus subtilis, yisR, degA
Topic(s):Biology
Presentation Type: Poster
Session: 3-11
Location: GEO - SUB
Time: 3:30