28th Annual Student Research Conference
Gene Expression Studies in the rld5409 Mutant of Maize
Gentry G. Fowler
Dr. Diane Janick-Buckner and Dr. Brent Buckner, Faculty Mentors
The recessive maize mutant rld5409 develops upwardly curled leaves, narrow leaf blades and ectopic ligules. Previous histological studies comparing wild-type and mutant leaves found a number of patterning defects, including reduced bulliform cells and leaf hairs on the adaxial surface of rld5409 leaves, patches of bulliform cells and leaf hairs on the abaxial surface of rld5409 leaves, as well as abnormal structure and patterning of stomata. Quantitative RT-PCR was performed on cDNA prepared from rld5409 and wild-type apex tissue to examine the expression of a number of genes involved in the establishment of leaf polarity. In addition, maize orthologs of rice genes involved in bulliform cell development were also examined.
Keywords: maize, leaf development, qRT-PCR, rpb4, gene expression
Topic(s):Biology
Presentation Type: Poster
Session: 3-11
Location: GEO-SUB
Time: 3:30