19th Annual Student Research Conference
Interdisciplinary
Microvascular PO2 (PO2m) in the Rat Ventricular Myocardium Using the Phosphorescence Quenching Method
Angela M. Ware*, Carl E. Giacchi, and Michael T. Paddock
Dr. Willaim Sexton (A.T. Still University) and Dr. Laura Fielden, Faculty Mentors
PO2m reflects the balance between oxygen delivery and oxygen consumption in a tissue. The purpose of this study was to develop a method to measure PO2m in the spontaneously beating rat heart at “rest” and during dobutamine infusion to increase myocardial work. PO2m was measured using phosphorescense quenching in the subepicardium of the left ventricular free wall for 30 s and during dobutamine infusion at 4.5, 9.0 and 18.0 µg/min to elevate contractility and myocardial oxygen consumption. Initial PO2m was 61 ± 5 mmHg. PO2m fell in a dose-dependent manner to 57 ± 5, 48 ± 5, and 38 ± 5 mmHg at 4.5, 9.0, and 18.0 µg/min of dobutamine, respectively. This method permits the measurement of PO2m in the beating rat heart, which can now be extended to interactions of disease and treatment paradigms.
Keywords: Heart, Oxygen, Dobutamine
Topic(s):Sigma-Xi
Presentation Type: Poster
Session: 60-81
Location: OP Lobby and Atrium
Time: 4:15