2021 Student Research Conference:
34th Annual Student Research Conference

IN VITRO EFFECTS OF SALINITY AND NORDIHYDROGUAIARETIC ACID ON Aβ 1-28 AGGREGATION


Mahan J. Hadjian
Dr. Brittany Hagenhoff, Faculty Mentor

Alzheimer’s Disease is a neurodegenerative disorder characterized by the formation of amyloidogenic plaques and tau protein fibrils. Recent literature has suggested that nordihydroguaiaretic acid (NA)—a polyphenol with potent antioxidant properties—may stifle the aggregation of amyloid-beta (Aβ) and serve in potential therapy. A monomerized sample of Aβ 1-28, a truncated fragment of the full peptide, was incubated with 25 μM NA. The aggregation of this treated sample was then monitored over the course of 14 days utilizing a Thioflavin T fluorescence assay and compared to untreated peptide. Similar analyses were performed for saline Aβ 1-28 samples. Aggregation was significantly attenuated in all treated samples, with this effect being most profound in the non-saline sample. Increasing sample salinity generally sped the rate of aggregation of Aβ 1-28 and nullified the therapeutic effect of NA, suggesting a possible limitation to NA use in Alzheimer’s therapy.

Keywords: Alzheimer's, Amyloid-ß, Thioflavin T, Nordihydroguaiaretic Acid, Salinity

Topic(s):Chemistry
Biochemistry and Molecular Biology

Presentation Type: Asynchronous Virtual Oral Presentation

Session: 3-22
Location: https://flipgrid.com/f86d186b
Time: 0:00

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