2017 Student Research Conference:
30th Annual Student Research Conference

Defining a Signaling Module for Vascular Development in Arabadopsis thaliana


Kendra N. Musmaker
Prof. Marisa Otegui (University of Wisconsin-Madison) and Dr. Brent Buckner, Faculty Mentors

Development of the plant vascular system involves a complex network of proteins. This network is thought to be a signaling module involving TETRASPANIN 1(tet1), VASCULAR COMPLEXITY AND CONNECTIVTY(vcc) and OCTOPUS(ops) which directs vascular development in conjunction with a Receptor-Like Kinase(RLK). Candidate RLKs include MORE LATERAL GROWTH 1(mol1), TRANSMEMBRANE LIKE KINASE(tmkl) and BRASSINOSTEROID RECEPTOR LIKE 2(brl2). To investigate this, F1 and F2 generations of vcc tmkl, vcc brl2 and ops mol1 were genotyped. One ops mol1 double mutant was identified; sample sizes were inadequate for definitive genotypes from the other double mutants. To further investigate the module, T-DNA mutants in ops, vcc, and vcc ops were grown in the presence of epibrassinolide. Brassinosteroids are plant hormones involved in cell elongation that function via RLKs, thus hypocotyl shortening was expected if a brassinosteroid-binding RLK is involved in the signaling module. Interestingly, hypocotyl lengthening was consistently seen for vcc ops.

Keywords: 

Topic(s):Biology

Presentation Type: Oral Paper

Session: 105-4
Location: MG 2001
Time: 8:45

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