27th Annual Student Research Conference
Mu-Seq Walking and Transcriptome Analysis of rld*5409: A Leaf Polarity Mutant in Maize
Kyler L. Carlson♦* and Lauren A. Choate
Dr. Diane Janick-Buckner and Dr. Brent Buckner, Faculty Mentors
Leaves of the recessive maize mutant rld*5409 demonstrate several defects including upward rolling of blade margins, reduced macrohairs and bulliform cells on the adaxial surface, and altered stomata structure and patterning. This project aimed to identify the causative gene for the rld*5409 phenotype and to assess how it affects leaf polarity networks. Mu-Seq Walking analysis indicates that the gene responsible for the rld*5409 phenotype resides on the distal end of chromosome 4. RNA-Seq libraries were prepared from young leaf primordia that were captured from rld*5409 and wild-type apices using laser microdissection. Using the Tuxedo workflow we identified a small set of genes that are differentially expressed between mutant and wild-type samples. One of these genes maps to the chromosome 4 interval identified by Mu-Seq Walking. Thus, a strong candidate gene for the rld*5409 phenotype has been identified and points to the network responsible for this phenotype.
Keywords: leaf polarity, Mu-Seq Walking, RNA-Seq, maize
Topic(s):Biology
Presentation Type: Poster
Session: 4-12
Location: GEO - SUB
Time: 3:30
♦ Indicates Truman Graduate Student