20th Annual Student Research Conference
Science
Identifying Zero Sequence Diversity Genes in Maize Using Temperature Gradient Capillary Electrophoresis (TGCE)
Julie M. Meyer
Dr. Mark Campbell, Faculty Mentor
Maize is one of the most important crops in the world. Genes that exhibit complete sequence conservation, or zero sequence diversity, may be key genes that are the difference between maize and all other wild relatives like teosinte. Among 24 inbred lines chosen, those displaying zero sequence diversity were retested and analyzed using temperature gradient capillary electrophoresis (TGCE). Of the 81 primer pairs designed from Maize Genomic Assembled Islands (MAGI) sequences, data was obtained for 67. Sixty-six were confirmed and one failed to be confirmed to exhibit zero sequence diversity. This indicates that the 66 sequences are highly conserved and might possibly be the genes that set maize apart from its wild relatives. Further testing of teosinte lines will show which of the zero sequence diversity genes in maize are responsible for the domestic phenotype of modern maize.
Keywords: maize, TGCE
Topic(s):Agricultural Science
Presentation Type: Poster
Session: 6-2
Location: OP Lobby
Time: 4:15 pm