20th Annual Student Research Conference
Science
Annotation and Analysis of Global Gene Expression Studies: Creation of a Maize Shoot Apical Meristem Expression Database
Zhian N. Kamvar*, Katy Browning, Ashleigh Fritz, and Eneda Hoxha
Dr. Brent Buckner and Dr. Diane Janick-Buckner, Faculty Mentors
As part of a larger project aimed at understanding the genes involved in shoot apical meristem function and leaf primordia formation, we have performed functional annotations of over 5,400 maize genes. These genes were identified as being differentially regulated in one of several microarray hybridization studies. We have created a database with a web-based interface to facilitate the functional annotation process, and to securely store our results (http://sam.truman.edu). Annotation of each differentially regulated gene includes the presentation and evaluation of BLAST and InterProScan searches. Assigning a gene to a functional category was done following an evaluation of the scientific literature and additional information found in a variety of databases. Information from these sources is stored in our annotator's notes section. The SAM database is fully searchable which facilitates the analysis and interpretation of the microarray experiments. Evaluation and interpretation of these data is ongoing and representative examples will be presented.
Keywords: Maize, Meristem, Gene Expression, Database, Annotation, Microarray
Topic(s):Biology
Presentation Type: Poster
Session: 7-4
Location: OP Lobby
Time: 4:15 pm